The DNA Sequence |
The mRNA Sequence |
TGGATAAACATTTCACCACTGTAAGGAAAATAATTCTTATTTCGATTGTCC
TTTTTACCCTTCTCGTTCGACTCATAGCTGAACACTCTAGAGAAAGAGGA GAAATACTAGATGTCAGTGATTAAGCAGGTAATGAAGACCAAGTTGCAC CTTGAGGGCACTGTCAATGGCCATGATTTTACGATCGAGGGTAAAGGTG AAGGCAAGCCGTACGAAGGGTTACAGCACATGAAAATGACAGTCACCA AAGGCGCGCCTCTGCCGTTTTCCGTTCATATTCTTACACCTAGCCACATG TATGGAAGCAAACCGTTTAATAAGTATCCAGCGGATATCCCAGACTACC ACAAACAGTCTTTTCCCGAAGGTATGTCTTGGGAGCGGTCGATGATTTT TGAAGATGGTGGCGTATGCACCGCCAGTAATCACTCCAGCATAAACTT GCAAGAGAACTGTTTCATCTATGATGTTAAATTTCATGGTGTGAACCTG CCTCCGGATGGGCCCGTAATGCAAAAAACCATTGCTGGATGGGAGCC GAGCGTGGAAACACTGTACGTGCGTGACGGGATGTTAAAAAGTGACA CTGCAATGGTTTTTAAACTGAAAGGAGGCGGTCATCATCGTGTTGATT TCAAAACGACGTATAAAGCCAAAAAACCTGTCAAGCTGCCAGAATTTCA TTTCGTTGAACATCGCCTGGAACTGACCAAACACGATAAAGATTTCACA ACTTGGGACCAGCAGGAGGCAGCCGAAGGCCATTTCTCACCGCTGCC GAAGGCTCTCCCACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAA GACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC |
UCUAGAGAAAGAGGAGAAAUACUAGAUGUCAGUGAUUAAGCAGGUAAUGAAGACCA
AGUUGCACCUUGAGGGCACUGUCAAUGGCCAUGAUUUUACGAUCGAGGGUAAAG GUGAAGGCAAGCCGUACGAAGGGUUACAGCACAUGAAAAUGACAGUCACCAAAGGC GCGCCUCUGCCGUUUUCCGUUCAUAUUCUUACACCUAGCCACAUGUAUGGAAGCA AACCGUUUAAUAAGUAUCCAGCGGAUAUCCCAGACUACCACAAACAGUCUUUUCCC GAAGGUAUGUCUUGGGAGCGGUCGAUGAUUUUUGAAGAUGGUGGCGUAUGCACCG CCAGUAAUCACUCCAGCAUAAACUUGCAAGAGAACUGUUUCAUCUAUGAUGUUAA AUUUCAUGGUGUGAACCUGCCUCCGGAUGGGCCCGUAAUGCAAAAAACCAUUGCU GGAUGGGAGCCGAGCGUGGAAACACUGUACGUGCGUGACGGGAUGUUAAAAAGU GACACUGCAAUGGUUUUUAAACUGAAAGGAGGCGGUCAUCAUCGUGUUGAUUUCA AAACGACGUAUAAAGCCAAAAAACCUGUCAAGCUGCCAGAAUUUCAUUUCGUUGAA CAUCGCCUGGAACUGACCAAACACGAUAAAGAUUUCACAACUUGGGACCAGCAGGA GGCAGCCGAAGGCCAUUUCUCACCGCUGCCGAAGGCUCUCCCA |
Amino Acid Squence |
MSVIKQVMKTKLHLEGTVNGHDFTIEGKGEGKPYEGLQHMKMTVTKGAPLPFSV
HILTPSHMYGSKPFNKYPADIPDYHKQSFPEGMSWERSMIFEDGGVCTASNHSSI NLQENCFIYDVKFHGVNLPPDGPVMQKTIAGWEPSVETLYVRDGMLKSDTAMVF KLKGGGHHRVDFKTTYKAKKPVKLPEFHFVEHRLELTKHDKDFTTWDQQEAAEG HFSPLPKALP |
Information About The Sequence
Promoter/Operator
The FecA promoter is repressed under replete conditions of iron. The promoter is regulated by FecI which is in turn regulated by FecA. These proteins are part of the ferric uptake system. FecA activates when ferrous dicitrate (iron) binds to its receptors which in turn turns the repressor on. In short - the promoter turns the gene on where there are low levels of iron. More on how this works can be found in the above link.
http://parts.igem.org/Part:BBa_J07019
Ribosome Binding Site (RBS)
This region is where the two ribosome units first bind to the mRNA to form the protein during translation, which occurs after the DNA undergoes transcription. This sequence is known to be efficient for and used in E. coli and sometimes other prokaryotes.
http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection
Structural Sequence
Only this sequence codes for the amino acid. Its purpose is to code for a pink protein, which can be seen by the naked eye. This sequence was optimized to be used in E. coli bacteria.
http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012
Terminator Sequence
This is a forward terminator sequence located at the end of the gene which stops RNA polymerase from elongating the mRNA strand during transcription.
http://parts.igem.org/wiki/index.php?title=Part:BBa_B0010
The FecA promoter is repressed under replete conditions of iron. The promoter is regulated by FecI which is in turn regulated by FecA. These proteins are part of the ferric uptake system. FecA activates when ferrous dicitrate (iron) binds to its receptors which in turn turns the repressor on. In short - the promoter turns the gene on where there are low levels of iron. More on how this works can be found in the above link.
http://parts.igem.org/Part:BBa_J07019
Ribosome Binding Site (RBS)
This region is where the two ribosome units first bind to the mRNA to form the protein during translation, which occurs after the DNA undergoes transcription. This sequence is known to be efficient for and used in E. coli and sometimes other prokaryotes.
http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection
Structural Sequence
Only this sequence codes for the amino acid. Its purpose is to code for a pink protein, which can be seen by the naked eye. This sequence was optimized to be used in E. coli bacteria.
http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012
Terminator Sequence
This is a forward terminator sequence located at the end of the gene which stops RNA polymerase from elongating the mRNA strand during transcription.
http://parts.igem.org/wiki/index.php?title=Part:BBa_B0010
Robetta Structure Query
Thanks to Robetta Query for folding and finding our protein! Click the image for a link to a 3D movable protein model!